Superoxide reduction by a superoxide reductase lacking the highly conserved lysine residue
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منابع مشابه
Role of protons in superoxide reduction by a superoxide reductase analogue.
Superoxide reduction by thiolate-ligated [FeII(SMe2N4(tren))]+ (1) involves two proton-dependent steps and a single peroxide intermediate, [FeIII(SMe2N4(tren))(OOH)]+ (2). An external proton donor is required, ruling out mechanisms involving H+ or H-atom abstraction from the ligand N-H. The initial protonation step affording 2 occurs with fairly basic proton donors (EtOH, MeOH, NH4+) in THF. Mo...
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Superoxide radical (O2.-) is the univalent reduction product of molecular oxygen and belongs to the group of the so-called toxic oxygen derivatives. For years the only enzymatic system known to catalyze the elimination of superoxide was the superoxide dismutase (SOD), which catalyzes dismutation of superoxide radical anions to hydrogen peroxide and molecular oxygen
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Desulfoferrodoxin is a small protein found in sulfate-reducing bacteria that contains two independent mononuclear iron centers, one ferric and one ferrous. Expression of desulfoferrodoxin from Desulfoarculus baarsii has been reported to functionally complement a superoxide dismutase deficient Escherichia coli strain. To elucidate by which mechanism desulfoferrodoxin could substitute for superox...
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PURPOSE To evaluate the age-induced changes in corneal endothelial morphology in mice lacking the cytosolic copper-zinc superoxide dismutase (SOD-1), the interstitial extracellular superoxide dismutase (SOD-3), or both of these SOD isoenzymes. METHODS The central corneal endothelial morphologies of old C57BL-6J wild type (n=19), SOD-1 null (n=16), SOD-3 null (n=15), and SOD1/3 null (n=11) mic...
متن کاملOrotate phosphoribosyltransferase from Corynebacterium ammoniagenes lacking a conserved lysine.
The pyrE gene, encoding orotate phosphoribosyltransferase (OPRTase), was cloned by nested PCR and colony blotting from Corynebacterium ammoniagenes ATCC 6872, which is widely used in nucleotide production. Sequence analysis shows that there is a lack of an important conserved lysine (Lys 73 in Salmonella enterica serovar Typhimurium OPRTase) in the C. ammoniagenes OPRTase. This lysine has been ...
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ژورنال
عنوان ژورنال: JBIC Journal of Biological Inorganic Chemistry
سال: 2014
ISSN: 0949-8257,1432-1327
DOI: 10.1007/s00775-014-1222-6